Journal: iScience

Article Title: Myeloid-intrinsic cell cycle-related kinase drives immunosuppression to promote tumorigenesis

doi: 10.1016/j.isci.2023.107626

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Article Snippet: BB755 anti-mouse CD4 (clone RM4-5) , BD Biosciences , 624391.

Techniques: Staining, Virus, Plasmid Preparation, shRNA, Recombinant, Expressing, Transfection, Magnetic Beads, Protein Extraction, Bicinchoninic Acid Protein Assay, Purification, Enzyme-linked Immunosorbent Assay, Software

Journal: iScience

Article Title: Mtb HLA-E-tetramer-sorted CD8 + T cells have a diverse TCR repertoire

doi: 10.1016/j.isci.2024.109233

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Article Snippet: mouse anti-human CD4 clone OKT4 BV650 , BD Bioscience , Cat. no: 750989; RRID: AB_2875058.

Techniques: Cell Isolation, Recombinant, Lysis, Transfection, Purification, Staining, Produced, TaqMan SNP Genotyping Assay, Plasmid Preparation, Software, Modification

Journal:

Article Title: Nef proteins encoded by human and simian immunodeficiency viruses induce the accumulation of endosomes and lysosomes in human T cells

doi:

Figure Lengend Snippet: CD4 surface expression by flow cytometry on control and Nef-expressing human T cell lines

Article Snippet: The 29-kDa molecular weight marker is indicated for each gel. table ft1 table-wrap mode="anchored" t5 Table 1 caption a7 Cell line Total mean fluorescence P3 OKT4 CEM-SS 4.7 188.4 CEM-SS/cat 5.4 105.9 CEM-SS/HIVfen 10.4 178.4 CEM-SS/SIV Nef 4.7 48.5 CEM-SS/HIV-1 NL43 Nef 4.9 21.1 CEM-SS/HIV-1 cons Nef 7.0 14.0 Jurkat 5.3 40.9 Jurkat/SIV Nef 3.0 16.9 SupT1 5.5 265.7 SupT1/SIV Nef 5.0 40.9 Open in a separate window Indirect immunofluorescent staining was performed using the anti-CD4 mAb OKT4 and the mouse IgG1 control mAb P3X63Ag8 (ATCC TIB9; unknown specificity).

Techniques: Expressing, Flow Cytometry, Control, Fluorescence